NUCLEOSIL

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure

  • Wide acceptance as routine packings for very different fields of chromatography.
  • One of the first spherical silicas used in HPLC.
  • An absolutely reliable choice in HPLC.
  • The largest variety of modified HPLC silicas available on the market.

Summary Of Available NUCLEOSIL Phases

Phase

Modification

% C

pH Range

Particle Sizes [µm]

USP Classification

RP phases

 

 

 

 

 

NUCLEOSIL® C18

medium density octadecyl, endcapped

15

2-8

3,5,7,10 µm

L1

NUCLEOSIL® C18 AB

crosslinkedoctadecyl, endcapped, high steric selectivity

25

1-9

5 µm

L1

NUCLEOSIL® C18 HD

high density octadecyl, endcapped

20

2-9

3,5 µm

L1

NUCLEOSIL® SiOH

Unmodified

2-8

5 µm

L3

NUCLEOSIL® C8 ec

medium density octyl, endcapped

8.5

2-8

5 µm

L7

NUCLEOSIL® C8

standard octyl, not endcapped

8.5

2-8

3,5,7,10 µm

L7

NUCLEOSIL® C8 HD

high density octyl, endcapped

13

2-8

5 µm

L7

NUCLEOSIL® NH2

aminopropyl -(CH2)3-NH2

3.5

2-8

5,10 µm

L8

NUCLEOSIL® SA

benzenesulfonic acid, strongly acidic cation exchanger (SCX)

6.5

2-8

5,10 µm

L9

NUCLEOSIL® CN

cyanopropyl (nitrile)

5

2-8

5,7,10 µm

L10

NUCLEOSIL® Phenyl

standard phenyl, not endcapped

8

2-8

5,7 µm

L11

NUCLEOSIL® SB

quaternary ammonium groups, strongly basic anion exchanger (SAX)

10

2-8

5,10 µm

L14

NUCLEOSIL® C2

dimethyl

3.5

2-8

7 µm

L16

NUCLEOSIL® OH

diol (dihydroxypropyl)

5

2-8

5,7 µm

L20

NUCLEOSIL® C4

medium density butyl, endcapped

2

2-8

5 µm

L26

NUCLEOSIL® C18 Nautilus

hydrophilic octadecyl, embedded polar group, endcapped

16

2-8

3,5 µm

L60

NUCLEOSIL® Protect I

special RP phase with protective polar group, endcapped

11

2-8

5 µm

 

NUCLEODUR®

NUCLEODUR® ® is a fully synthetical type B silica (silica of 3rd generation) offeringhighly advanced physical properties:

  • Totally spherical particle shape
  • Outstanding surface microstructure
  • High pressure stability
  • Low metal content

Summary Of Available NUCLEODUR® Phases

Phase

Modification

% C

pH Range

Particle Sizes [µm]

USP Classification

NUCLEODUR®  C18 Gravity

Octadecyl, high density coating, multi-endcapping

18

1–11

1.8, 3, 5

L1

NUCLEODUR®  C18 Gravity SB

Monomeric octadecyl modification, extensive endcapping;

13

1-9

1.8, 3, 5

L1

NUCLEODUR®  C18 HTec

Octadecyl, high density coating, high capacity, multi-endcapping

18

1-11

1.8, 3, 5, 7, 10

L1

NUCLEODUR®  C18 Isis

Octadecyl, specially cross-linked modification, endcapped

20

1-10

1.8, 3, 5

L1

NUCLEODUR®  C18 Pyramid

Octadecyl with polar endcapping

14

1-9

1.8, 3, 5

L1

NUCLEODUR®  PolarTec

Octadecyl with embedded polar group

17

1-9

3,5

L1 L60

NUCLEODUR®  C18 ec

Octadecyl, medium density, endcapping

17.5

1–9

3, 5 and larger

L1

NUCLEODUR®  SiOH

Unmodified NUCLEODUR® ®

 

3, 5 and larger

L3

NUCLEODUR®  C8 Gravity

Octyl, high density coating, multi-endcapping

11

1-11

1.8, 5

L7

NUCLEODUR®  C8 ec

Octyl, medium density, endcapping

10.5

1-9

3,5

L7

NUCLEODUR®  NH2/NH2-RP

Aminopropyl for NP and RP separations

2.5

2–8

3, 5

L8

NUCLEODUR®  CN/CN-RP

Cyano (nitrile) for NP and RP separations

7

1-8

3,5

L10

NUCLEODUR® π²

Hydrophobic phase with alternative selectivity compared to classical C18 modifications

17

3-10

5

L11

NUCLEODUR®  PFP

Pentafluorophenyl-propyl with multi-endcapping

8

1-9

3,5

L43

NUCLEODUR®  Sphinx RP

Bifunctional, balanced ratio of propylphenyl and octadecyl, endcapping

15

1-10

1.8, 3, 5

L1 L11

NUCLEODUR®  HILIC

Ammonium – sulphonic acid

7

2–8.5

1.8, 3, 5

 

 

NUCLEOSHELL®

  • Solid core of silicon dioxide,homogeneous shell of porous silica.
  • Highest efficiency compared to traditional totally porous materials.
  • Pore size 90 Å; particle size 2.7 µm (core 1.7 µm); specific surface 130 m2/g.
  • Lower back pressure also allows use on conventional LC systems.
  • Pressure stability up to 600 bar.

Phase

Modification

% C

pH Range

Particle Sizes [µm]

USP Classification

NUCLEOSHELL®  RP 18

Octadecyl modification, multi-endcapped

7.8 for 2.7µm

6.1  for 5µm

1–11.0

2.7 µm, 5 µm

L1

NUCLEOSHELL®  RP 18plus

polar octadecyl modification, multi-endcapped

5.7 for 2.7µm

4.4 for 5µm

2-9

2.7 µm, 5 µm

L1

NUCLEOSHELL®  Bluebird RP18

Special octadecyl core-shell phase with hydrophilic endcapping

5

1-8

2.7 µm (core 1.7 µm)

 

L1

NUCLEOSHELL®  Phenyl-Hexyl

phenyl-hexyl modification, multi-endcapped, 4.5 % C

4.5

1-10

2.7 µm

L11

NUCLEOSHELL®  HILIC

Ammonium – sulfonic acid modification

1.3

2–8.5

2.7 µm

 

NUCLEOSHELL®  PFP

Pentafluorophenyl modification,  multi-endcapped

~ 3

1.0–9.0

2.7 µm

L43

 

Columns for Special Applications

Separation / Mechanism

Recommended Column

Specification Of The Phase

USP Classification

Environmental analysis

anion exchangechromatography of inorganic anions

NUCLEOGEL®Anion I

strongly basic polymer-based anion exchanger

 

NUCLEOSIL®Anion II

strongly basic silica-based anion exchanger

 

RP chromatography of PAHs

NUCLEODUR® ®C18PAH, 3µm

NUCLEODUR® ®polymer-coated with C18 groups

L1

NUCLEOSIL®100-5 C18PAH

NUCLEOSIL®100 polymer-coated with C18groups

L1

Enantiomer separation

based on formation of inclusion complexes

NUCLEODEX α-PM, β-PM, γ-PM and β-OH

silica-based permethylated and underivatisedcyclodextrin phases

L45

based on polar and π-π interactions

NUCLEOCEL DELTA

silica-based modified cellulose phases

L40

NUCLEOCEL ALPHA

silica-based modified amylosephases

L51

based on ligand exchange

NUCLEOSIL®CHIRAL-1

covalently bonded amino acid – Cu(II) complexes

L32

based on charge-transfer-, dipole-dipole interactions and others

NUCLEOSIL® CHIRAL-2 NUCLEOSIL®CHIRAL-3

silica-based brush type phases

L36

based on enantioselective bindingto chiral protein surface structures

RESOLVOSIL BSA-7

silica-based protein phase (BSA)

 

Biological macromolecules

anion exchangechromatography of proteins and peptide

NUCLEOSIL®4000-7 PEI

silica-based polymeric poly ethyleneimine network

 

anion exchange chromatography of oligonucleotides and nucleic acids

NUCLEOGEN®DEAE

silica-based DEAE anion exchanger

 

anion exchange chromatography
of peptides, large proteins and
oligonucleotides

NUCLEOGEL®SAX

polymer-based strongly basic anion
exchanger

L23

cation exchange chromatographyof proteins, peptides and carbohydrates

NUCLEOGEL®SCX

polymer-based strong cation exchanger

L22

reversed phase chromatography of proteins, peptides and oligonucleotides

NUCLEOSIL®MPN

monomerically bonded alkyl chains on silica

L1L26

NUCLEOSIL®PPN

polymerically bonded alkyl chains on silica

L1

NUCLEOGEL®RP 300

polystyrene – divinylbenzene polymer

L21

reversed phasechromatography of small molecules

NUCLEOGEL®RP 100

small pore macroporous PS-DVB polymer

L21

Food analysis – Sugars

RP chromatography of mono- and oligo-saccharides

NUCLEOSIL®Carbohydrate

silica-based special amino phase

L8

separation of sugars, alcohols, organic acids based on ion exclusion, ion exchange, size exclusion, ligand exchange, NP and RP effects

NUCLEOGEL® SUGAR 810 H

PS-DVB resins with sulphonic acid
modification in H form

L17

NUCLEOGEL® SUGAR 810 Ca

PS-DVB resins with sulphonic acid modification in Ca form

L19

separation of sugars, alcohols, organic acids based on steric exclusion, ligand exchange and partition effects

NUCLEOGEL®ION 300 OA

PS-DVB resins with sulphonic acid modification in H form

L17

NUCLEOGEL® SUGAR Ca

PS-DVB resins with sulphonic acid modification in Ca form

L19

NUCLEOGEL® SUGAR Pb

PS-DVB resins with sulphonic acid modification in Pb form

L34

NUCLEOGEL® SUGAR Na

PS-DVB resins with sulphonic acid
modification in Na form

L58

Gel permeation chromatography (GPC)

water-insoluble compounds

NUCLEOGEL®GPC

polystyrene – divinylbenzene polymer

 

 

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