TLC Micro Sets

TLC micro-set A for beginners

This kit contains all chemicals and accessories for the following separations:

• separation of the fat-soluble (lipophilic) dye mix-ture 1: butter yellow, indophenol, sudan blue II, sudan red G
• separation of a mixture of anthraquinone dyes (test dye mixture 2): blue 1, blue 3, green, green blue, red, violet 1, violet 2
• separation of a mixture of food dyes (test dye mixture 3): brilliant black BN (E151), fast red E, erythrosine (E127), yellow orange S (sunset yellow CFC, E110), naphthol red S, ponceau 4 R (E124), tartrazine (E102)
• separation of dyes from felt tip pens

Visualisation Reagents

Visualisation reagents

• a small selection of frequently used spray reagents for postchromatographic detection reactions in TLC suited for spraying or dipping TLC plates
• a detailed description of many more detection procedures for TLC is available on request

Adsorbents for TLC

Silica

pore size 60 Å, pore volume 0.75 ml/g, specific surface (BET) ~ 500 m2/g, pH of a 10 % aqueous suspension 7.0

Silica G

standard grade, particle size 2 – 20 μm, Fe < 0.02 %, Cl < 0.02 %, 13 % gypsum as binder, supplied with or without fluorescence indicator UV254

Silica N

standard grade, particle size 2 – 20 μm, Fe < 0.02 %, Cl < 0.02 %, no binder, supplied with or without fluorescence indicator UV254

Silica G-HR

high purity grade, particle size 3 – 20 μm, Fe < 0.002 %, Cl < 0.008 %, gypsum as binder, supplied without fluorescence indicator

Silica P

preparative grade, particle size 5 – 50 μm, Fe < 0.02 %, Cl < 0.02 %, organic binder, supplied with fluorescence indicator UV254

Silica P with gypsum

preparative grade, particle size 5 – 50 μm, Fe < 0.02 %, Cl < 0.02 %, gypsum as binder, supplied with fluorescence indicator UV254

Aluminium oxide adsorbents for TLC

pore size 60 Å, specific surface (BET) ~ 200 m2/g

• Aluminium oxide G ~ 10 % gypsum as binder, supplied with or without fluorescence indicator
• Aluminium oxide N no binder, supplied without fluorescence indicator

Polyamide adsorbents for TLC

• Polyamide 6 = nylon 6 = perlon = ε-aminopolycaprolactame

Cellulose MN 301 native fibrous cellulose

• fibre length (95%) 2 – 20 μm, average degree of polymerisation 400 – 500, specific surface acc. to Blaine 15000 cm2/g
• Cellulose MN 301: native fibrous cellulose, standard grade
  ≤ 20 ppm Fe, 6 ppm Cu, 7 ppm P, CH2Cl2 extract ≤ 0.25%, residue on ignition at 850 °C ≤ 1500 ppm
• Cellulose MN 301 HR: fibrous cellulose, high purity grade, acid-washed and defatted
  ≤ 2 ppm Fe, 1 ppm Cu, CH2Cl2 extract ≤ 0.025%, residue on ignition at 850 °C ≤ 200 ppm recommended for quantitative investigations, e. g. for separation of carbohydrates with subsequent IR spectroscopy or separation of phosphoric acids, phosphates etc.
• Cellulose MN 301 A: special grade for the 32P postlabelling procedure
  ≤ 20 ppm Fe, ≤ 6 ppm Cu, ≤ 7 ppm P, CH2Cl2 extract ≤ 0.01%, residue on ignition at 850 °C ≤ 500 ppm free of lactobacili contaminations; not impregnated with PEI, but designed for impregnation and coating by the user

Fluorescent indicators

UV indicators with efficient radiation for short-wave as well as long-wave UV ranges
UV254: manganese activated zinc silicate with absorption maximum at 254 nm; green fl uorescence; relatively susceptible towards acids; thus its fluorescence can be completely quenched by acidic solvents
UV366: inorganic fluorescent pigment with absorption maximum at 366 nm; blue fluorescence