Solid Phase Extraction
SPE Vacuum Manifold
VacElut 20 Manifold
VacElut20
Corrosion-resistant VacElut 20 permits simultaneous processing of up to 20 SPE cartridges. The manifold’s clear glass base allows careful monitoring of the entire sample collection process.
The VacElut 20 vacuum control valve, vacuum gauge, and quick release valve are mounted on the lid, away from the corrosive solvent stream and within convenient reach.
VacElut SPS 24 Manifold
 VacElut SPS24
- The VacElut SPS 24 allows simultaneous processing of up to 24 SPE cartridges.
- VacElut SPS 24 is made from durable, solvent-resistant materials and engineered
to last.
- The glass sides allow easy viewing of the entire sample collection process
The ultimate feature of the SPS 24 manifold is its waste diversion funnel, which enables all steps of the SPE procedure to be completed without removing the lid. Since the collection rack is placed inside the unit before extraction begins, splash back and cross contamination are eliminated, while hazardous waste and biohazard exposure are minimized.
Complete with replacement stainless steel delivery tips for maximum extract purity, the VacElutSPS 24 system also includes a vacuum controller/release, collection rack, and port sealing plugs.
Check Vacuum Pumps for Manifold.
SPE Columns Format
SPE Cartridges/Columns are available in various formats for varied applications.
CHROMABOND® polypropylene columns
- PP columns with PE filter elements
- different sizes from 1, 3, 6 up to 150 ml
- adsorbent weights from 20 mg to 50 g
- male luer tip as exit
- compatible with most robots (e. g. Gilson ASPEC™, Caliper AutoTrace®, …)
CHROMABOND®glass columns
- glass columns with chemically very inert glass fibre filter elements (nominal pore size 1 µm)
- two different sizes: 3 and 6 ml
- available with all CHROMABOND® phases
- excludes any influence from the column material (e. g. plasticizers, …)
CHROMAFIX®cartridges
- PP cartridges with PE filter elements
- three different sizes with different adsorbent weights: Small (0.4 ml), Medium (0.8 ml), Large (1.8 ml)
- female Luer tip at the inlet, male Luer tip as exit
- offers alternative way of handling using positive
- pressure by syringes or peristaltic pumps
- especially suited for convenient solid phase extraction of small sample volumes
CHROMABOND®LV columns
- large volume PP columns with PE filter elements
- three different adsorbent weights (100, 200 and 500 mg)
- funnel-shaped reservoir with 15 ml volume
- especially for clinical samples - the whole sample (e. g. urine, serum, blood) can be applied to the column in one step
- can be directly used in the Zymate®lab robots of Zymark
CHROMABOND®MULTI 96 SPE in 96-well format
- 96-well polypropylene plates with PE filter elements
- cavity volume 1.5 ml
- adsorbent weights from 25 to 100 mg
- supplied with any CHROMABOND® SPE adsorbents
- for simultaneous preparation of 96 samples
- easy method transfer from CHROMABOND®columns or CHROMAFIX® cartridges to CHROMABOND®MULTI 96
SPE Columns
SPE columns are available for
- Reverse Phase
- Normal Phase
- Ion Exchange Phase
- Phase for Special Applications
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| Reverse phases for SPE |
| Code |
Matrix |
Particle size |
Pore size |
Specification |
| HR-X |
PS/DVB |
45 µm / 85 µm |
55–60 Å |
spherical particles |
| EASY |
PS/DVB |
80 µm |
50 Å |
polar, bifunctional modification |
| HR-P |
PS/DVB |
50–100 µm |
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| PS-RP |
PS/DVB |
100 µm |
100 Å |
for removal of organic components from water |
| C18 ec |
silica |
45 µm |
60 Å |
octadecyl modification, endcapped |
| C18 ec f |
silica |
100 µm |
60 Å |
as above, fast flow |
| C18 |
silica |
45 µm |
60 Å |
octadecyl modification, not endcapped |
| C18 f |
silica |
100 µm |
60 Å |
as above, fast flow |
| C18 PAH |
silica |
45 µm |
60 Å |
special octadecyl phase, for enrichment of PAHs from water |
| C18 Hydra |
silica |
45 µm |
60 Å |
octadecyl modification, not endcapped, for polar analytes |
| C8 |
silica |
45 µm |
60 Å |
octyl modification |
| C4 |
silica |
45 µm |
60 Å |
butyl modification |
| C2 |
silica |
45 µm |
60 Å |
dimethyl modification |
| C6H11 ec |
silica |
45 µm |
60 Å |
cyclohexyl modification, endcapped |
| C6H5 |
silica |
45 µm |
60 Å |
phenyl modification |
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| Normal phases for SPE |
| Code |
Matrix |
Particle size |
Pore size |
Modification |
| SiOH |
silica |
45 µm |
60 Å |
unmodified |
| NH2 |
silica |
45 µm |
60 Å |
aminopropyl |
| OH (Diol) |
silica |
45 µm |
60 Å |
diol |
| CN |
silica |
45 µm |
60 Å |
cyano (nitrile) |
| Alox A |
aluminium oxide, acidic |
60–150 µm |
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| Alox N |
aluminium oxide, neutral |
60–150 µm |
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| Alox B |
aluminium oxide, basic |
60–150 µm |
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| Florisil® |
magnesium silicate |
150–250 μm |
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| PA |
polyamide 6 |
40–80 μm |
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| Ion exchange phases for SPE |
| Code |
Matrix |
Particle size |
Pore size |
Specification |
| HR-XC |
PS/DVB |
45 µm / 85 µm |
60 Å |
65–75 Å benzenesulfonic acid modification, very strong mixed mode cation exchanger (MCX) |
| HR-XA |
PS/DVB |
45 µm / 85 µm |
55–65 Å |
quaternary ammonium modification, very strong mixed mode anion exchanger (MAX) |
| HR-XCW |
PS/DVB |
45 µm / 85 µm |
50–60 Å |
carboxylic acid modification, vweak mixed mode cation exchanger (WCX) |
| HR-XAW |
PS/DVB |
45 µm / 85 µm |
55–65 Å |
secondary and tertiary ammonium modification,
weak mixed mode anion exchanger (WAX) |
| SA |
silica |
60–150 µm |
60 Å |
benzenesulfonic acid modification, strongly acidic cation exchanger (SCX) |
| SB |
silica |
60–150 µm |
60 Å |
quaternary ammonium modification, strongly basic anion exchanger (SAX) |
| PCA |
silica |
45 μm |
60 Å |
propylcarboxylic acid modification, weakly acid cation exchanger (WCX) |
| PSA |
silica |
45 μm |
60 Å |
propylsulfonic acid modification, very strong cation exchanger |
| PS-OH– |
PS/DVB |
100 μm |
100 Å |
PS/DVB anion exchanger, OH– form for removal or concentration of anions from water, increasing the pH value in acidic samples |
| PS-H+ |
PS/DVB |
100 μm |
100 Å |
PS/DVB cation exchanger, H+ form for removal or concentration of cations from water, decreasing the pH value of basic samples |
| PS-Mix |
PS/DVB |
100 μm |
100 Å |
mixture of PS-H+ and PS-OH– for desalting of water |
| PS-Ag+ |
PS/DVB |
100 μm |
100 Å |
PS/DVB cation exchanger, Ag+ form for removal of halide ions from water |
| PS-Ba2+ |
PS/DVB |
100 μm |
100 Å |
PS/DVB cation exchanger, Ba2+ form for removal of sulfate ions from water |
| SPE phases for special applications |
| CHROMABOND® |
Matrix
| Modification |
Application |
| Pharmaceutical analysis |
| Drug |
silica |
bifunctional, C8 / SA (strong cation exchanger, benzenesulfonic acid) |
acidic, neutral and basic drugs like opiates, amphetamines |
| Drug II |
silica |
bifunctional, C8 / SB (strong anion exchanger, quaternary amine) |
THC and THC derivatives, acidic analytes from biological fluids |
| Crosslinks |
cellulose |
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collagen crosslinks from urine |
| Tetracycline |
silica |
special C18 phase |
tetracyclines from biological samples |
| Environmental analysis |
| HR-P-AOX |
PS/DVB |
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AOX from waters with high salt loads (DIN 38409 - H22) |
| C18 PAH |
silica |
special octadecyl phase |
PAHs from water |
| CN/SiOH |
silica |
cyanopropyl silica and unmodified silica |
PAHs from soil acc. to EPA |
| NH2/C18 |
silica |
aminopropyl silica and octadecyl silica |
PAHs from water containing humic acids |
| Na2SO4/Florisil® |
sodium sulfate / Florisil® |
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determination of hydrocarbons from water according to DIN H-53 / ISO DIS 9377-4 |
| SA/SiOH |
silica |
strong cation exchanger and unmodified silica |
PCBs from waste oil |
| SiOH-H2SO4/SA |
silica |
sulfuric acid impregnated silica and strong cation exchanger |
PCBs from oil acc. to the german standard DIN 51527 |
| NAN |
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sodium sulfate (N) and SiOH/AgNO3 (A) |
PCBs from sludge |
| Food analysis |
| ABC18 |
silica |
octadecyl and ion exchange functions |
extraction of acrylamide from food |
| Diamino |
silica |
primary and secondary amine functions (PSA) |
pesticides in food by the QuEChERS method |
| QuEChERS |
silica |
QuEChERS mixes: individually weighed and premixed buffer and extraction mixtures, specially composed for different sample matrices |
pesticides in food by the QuEChERS method |
| Other special phases |
| Dry (Na2SO4) |
anhydrous sodium sulfate |
drying of organic solutions |
| XTR |
kieselguhr |
liquid-liquid extraction |
| PTL / PTS |
special membranes |
separation of two-phase mixtures without separation funnel |
CHROMABOND®HR-Xpert
The innovative concept of five polymer-based RP and mixed-mode ion exchange phases for SPE:
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- CHROMABOND®HR-X hydrophobic PS/DVB copolymer
- CHROMABOND®HR-XC strong mixed-mode cation exchanger
- CHROMABOND®HR-XA strong mixed-mode anion exchanger
- CHROMABOND®HR-XCW weak mixed-mode cation exchanger
- CHROMABOND®HR-XAW weak mixed-mode anion exchanger
CHROMABOND®HR-Xpert is the perfect combination for all tasks in sample preparation
These innovative SPE phases offer:
state-of-the-art spherical polymer
- broad spectrum of applications with special suitability for enrichment of pharmaceuticals from biological matrices
- ideal flow properties due to low content of particulate matter
optimised pore structure and high specific surface
- highloadability and outstanding elution properties
- low solvent consumption
- rapid, economical analyses
high-purityadsorber material
- allows highest reproducibility with extremely low blind values
- reliable analyses at ultra trace level
- no method adaptation for new batches necessary
SPE Method Development Kits
SPE method development kits are available for
 Chromabond Packages
- Investigating the best separation mechanism for a clean-up procedure
- Selecting the optimum RP phase for clean-up procedure
- Selecting the optimum polar phase for a clean-up procedure
- Selecting the optimum ion exchange for a clean-up procedure
- Phase selection for clean-up procedures for environmental samples.
Automated and on-line SPE
- Ready-to-use EC columns or ChromCart® cartridges for online-SPE
Standard dimensions 20 x 2 mm and 20 x 4 mm
Packed with CHROMABOND® HR-Xpert phases (15 µm particles) or with NUCLEODUR® C18 ec, C8 ec, CN (20 µm particles)
- Special SPE columns already equipped with special caps and needles to be used in the SPE unit of theGerstel MultiPurposeSampler (MPS), available in 1, 3 and 6 mL
- Columns for Gilson ASPEC™ systems are ready-to-use assembled with caps. In addition to the columns and phases, all 1, 3 and 6 mL CHROMABOND® polypropylene columns can be supplied assembled with ASP caps.
 EC columns  CC columns  SPE cartridges for the Gerstel MPS system
 The Gerstel
MPS system
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