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| HPLC Columns |
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| Silica Manufacturer |
Brand |
| CERI |
L-ODS |
| Chemco
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Chemcosorb |
| ES Industries
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AquaSep, Chromegabond, FluoroSep, Gammabond, ProTec |
| Macherey-Nagel
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Nucleodur, Nucleosil, Nucleocel, Nucleodex, Nucleogen, Polygosil, Resolvosil |
| Polymer Laboratories
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PLRPS , Hi-Plex, PL Gel, PolyPore, ResiPore, MesoPore, OligoPore |
| PolyLC
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PolySULFOETHYL Aspartamide, PolyPROPYL Aspartamide, PolyETHYL Aspartamide, PolyMETHYL Aspartamide, PolyWAX LP, PolyGLYCOPLEX, PolyCAT A
Columns for Protein, Peptides, Carbohydrates
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| Shinwa Chemical Industries
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Ultron |
| Sumika Chiral
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SumiChiral OA |
| Varian
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Polaris, Pursuit, Microsorb |
| Zirchrom
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Zirchrom PBD |
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| Column Type |
Stationary Phase/Separation Process |
Construction Material |
| Analytical Column |
Normally Phase |
Steel - Stainless |
| Semi Preparative Column
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Reversed Phase |
Polymer |
| Preparative Column
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Ion Exchange |
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| UPLC Column
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Gel Permeation |
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| LC MS Column
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Enantiomer |
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| Guard Column
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Polymer |
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| Column Lengths (mm) |
Internal Diameter (mm) |
Particle Size (µm) |
| 30, 50,75,100,125,150,
200,250,300,500mm
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1,2,3,4,4.6,10,20,32,40,50,80mm |
1.8,2.4,2.8,3,5,7,10µm |
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| Require application support for HPLC column selection, contact us |
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| ION Exchange HPLC Columns |
| Nucleosil SA |
- strongly acidic cation exchangers (SCX) with benzenesulphonic acid modification
- capacity ~ 1 meq/g
- pH stability at 20 °C: 2 – 8
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| Nucleosil SB
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- strongly basic anion exchangers (SAX) with quaternary ammonium modification
- capacity ~ 1 meq/g
- pH stability at 20 °C: 2 – 8
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| Nucleogel SAX (USP L23)
anion exchange of biological macromolecules |
- polymer-based strongly basic anion exchanger -N+(CH3)3, gel matrix quaternised PEI; particle size 8 μm,
- available pore sizes 1000 Å and 4000 Å ∙ pH working range 1 – 13, max. working pressure 200 bar
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| Nucleogel SCX (USP L22)
cation exchange of biological macromolecules |
- polymer-based strongly acidic cation exchanger -SO3–, hydrophilic gel matrix; particle size 8 μm,
- available pore sizes 1000 Å and 4000 Å ∙
- pH working range 1 – 13, max. working pressure 200 bar
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| NUCLEOGEL® Anion I
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- strongly basic polymer-based anion exchanger, particle size 10 μm
- pH stability: 1 – 14
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| NUCLEOSIL® Anion II
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- base material NUCLEOSIL® silica, particle size 10 μm, pore size 300 Å
- strongly basic anion exchanger, exchange capacity 50 μeq/g
- pH stability 2 – 7.5
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Ion Exchange HPLC Columns for
Proteomics, Phosphopeptides, Protein Variants, Metabolomics, HILIC, SEC
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| PolyGLYCOPLEX™ |
For Complex Carbohydrates |
| PolyCAT A™
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For Cation-Exchange of Proteins |
| PolySULFOETHYL Aspartamide™
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For Cation-Exchange of Peptides |
| PolyPROPYL Aspartamide™
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For Hydrophobic Interaction Chromatography (HIC) of Proteins and Peptides |
| PolyETHYL Aspartamide™
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For Hydrophobic Interaction Chromatography (HIC) of Proteins and Peptides |
| PolyMETHYL Aspartamide™ |
For Hydrophobic Interaction Chromatography (HIC) of Proteins and Peptides |
| PolyWAX LP™
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For Anion-Exchange of Proteins |
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Hydrophilic Interaction Chromatography (HILIC)
Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC)
Size Exclusion Chromatography (SEC)
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| Chiral HPLC Columns |
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| Column Name |
USP |
Mode |
Description |
| Macherey Nagel, Germany |
| Nucleocel Alpha
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L-51 |
NP, RP |
amylose tris-(3,5-dimethylphenylcarbamate) |
| Nucleocel Delta |
L-40 |
NP, RP |
cellulose tris-(3,5-dimethylphenylcarbamate) |
| Nucleosil Chiral 1 |
L-32 |
- |
L-hydroxyproline / Cu2+ complexes |
| Nucleosil Chiral 2 |
- |
- |
N-(3,5-dinitrobenzoyl)-Dphenylglycine |
| Nucleosil Chiral 3 |
L-36 |
- |
Chiral selector is the optical antipode of the CHIRAL-2 phase |
| Nucleodex α- PM |
- |
- |
permethylated α-cyclodextrin (R = CH3; n = 1) |
| Nucleodex γ- PM |
- |
- |
permethylated γ-cyclodextrin (R = CH3; n = 3) |
| Nucleodex beta OH |
L-45 |
- |
β-cyclodextrin (R = H; n = 2) |
| Nucleodex beta PM |
L-45 |
- |
permethylated β-cyclodextrin (R = CH3; n = 2) |
| Resolvosil BSA 7 |
- |
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bovine serum albumin (BSA) |
| Shinwa Chemical, Japan |
| ULTRON ES OVM |
L-57 |
- |
Chiral separation column immobilized with ovomucoid which is strong protein for denaturation (US PATENT 6027648) |
| Sumi Chiral, Japan |
| Sumi Chiral OA-2000 |
- |
NP |
(R)-phenylglycine |
| Sumi Chiral OA-2500 |
- |
RP |
(R)- 1-naphthylglycine |
| Sumi Chiral OA-3100 |
- |
NP, RP |
(S), Valline |
| Sumi Chiral OA-3200 |
- |
NP, RP |
(S) – tert-leucine |
| Sumi Chiral OA-3300 |
- |
NP, RP |
(R )- phenylglycine |
| Sumi Chiral OA-4000 |
- |
NP |
(S)-Valine (S)-1(alpha-naphtyl)ethylamine |
| Sumi Chiral OA-4100 |
- |
NP |
(S)-valine (R ) -1(alpha-naphtyl)ethylamine |
| Sumi Chiral OA-4400 |
- |
NP |
(S)-proline (S)-1(alpha-naphtyl)ethylamine |
| Sumi Chiral OA-4500 |
- |
NP |
(S)-prolie ( R)-1(alpha-naphtyl)ethylamine |
| Sumi Chiral OA-4600 |
- |
NP |
(S) – tert-leucine (S) -1-(alpha-naphthyl)ethylamine |
| Sumi Chiral OA-4700 |
- |
NP |
(S) – tert-leucine ( R) -1-(alpha-naphthyl)ethylamine |
| Sumi Chiral OA-4800 |
- |
NP |
(S)-indoline-2-carboxylic acid (S)-1- (alpha-naphthyl) ethylamine |
| Sumi Chiral OA-4900 |
- |
NP |
(S)-indoline-2-carboxylic acid (R)-1- (alpha-naphthyl) ethylamine |
| Sumi Chiral OA-5000 |
- |
RP |
(D)-penicillamine |
| Sumi Chiral OA-6000 |
- |
RP |
(L)-tartaric acid (S)-1-(alpha-naphtyl)ethylamine |
| Sumi Chiral OA-6100 |
- |
RP |
(L)-tartaric acid, (S)-valine ( R) -1-(alpha-naphthyl) ethylaminie |
| Sumi Chiral OA-7000 |
- |
RP |
Beta cyclodextrin with novel spacer |
| Sumi Chiral OA-8000 |
- |
NP,RP |
Chiral pseudo 18 –crown-6 ether |
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| ** Guard column, Analytical column & Preparative Chiral HPLC columns available |
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| All respective trademarks acknowledged. |
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